Inorganic polyphosphates enhances nucleus pulposus tissue formation in vitro
نویسندگان
چکیده
منابع مشابه
Cartilage tissue enhances proteoglycan retention by nucleus pulposus cells in vitro.
OBJECTIVE To investigate the effect of cartilage on nucleus pulposus (NP) tissue in an in vitro model. METHODS Cells were isolated from bovine NP or articular cartilage and allowed to form tissue in vitro. The NP tissue was grown either alone or in the presence of cartilage tissue (coculture) for up to 4 weeks and examined for histologic appearance, gene expression, and biochemical compositio...
متن کاملNotochordal cell rich nucleus pulposus tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human MSCs
pulposus phenotype in human MSCs +Purmessur, D; Godburn K; Abbott, RD: Schek RM; Iatridis JC +University of Vermont, Burlington VT Senior author [email protected] INTRODUCTION Mesenchymal stem cells (MSCs) are an attractive cell source for IVD repair, yet the means of differentiating these cells to a nucleus pulposus (NP) phenotype is not clear and an area of high priority. Notochordal cel...
متن کاملCorrection: The new world of inorganic polyphosphates.
Figure 1 Model of two independent PTMs, serine-pyrophosphorylation and lysine-polyphosphorylation Protein pyrophosphorylation is the transfer of the b-phosphate group of the pyrophosphate moiety of IP7 on to a pre-phosphorylated serine residue (left) [16,17]. Conversely, protein polyphosphorylation is the covalent attachment of a chain of inorganic polyP on to a lysine residue dependent on the ...
متن کاملNucleus pulposus explant culture model.
Many of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro with or without chondrogenic reagents for differen...
متن کاملAn in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue
INTRODUCTION The decreased disc height characteristic of intervertebral disc (IVD) degeneration has often been linked to low back pain, and thus regeneration strategies aimed at restoring the disc extracellular matrix and ultimately disc height have been proposed as potential treatments for IVD degeneration. One such therapy under investigation by a number of groups worldwide is the use of auto...
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ژورنال
عنوان ژورنال: Journal of Orthopaedic Research
سال: 2016
ISSN: 0736-0266
DOI: 10.1002/jor.23288